Composite
Part:BBa_K2637029:Design
Designed by: Haoqian Zhao Group: iGEM18_Tianjin (2018-10-05)
KaiB cassette with PGK1 Promoter and PGK1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 750
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PGK1 Promoter + KaiB + PGK1 Terminator. And KaiB has been optimized in yeast.
Source
PGK1P and PGK1T are the promoter and terminator of yeast. KaiB is from Synechococcus elongatus PCC7942.