Composite

Part:BBa_K2637029:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)


KaiB cassette with PGK1 Promoter and PGK1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PGK1 Promoter + KaiB + PGK1 Terminator. And KaiB has been optimized in yeast.

Source

PGK1P and PGK1T are the promoter and terminator of yeast. KaiB is from Synechococcus elongatus PCC7942.

References